The study consisted of two arms. The retrospective arm consisted of 25 fresh frozen tissues from GBM patients that were tested for CMV by real-time PCR. Tissue microarrays from 70 HGG patients were tested by immunohistochemistry (IHC) for the presence of CMV proteins. 20 formalin-fixed paraffin-embedded GBM tissues were tested by IHC and chromogenic in situ hybridization, targeting specific CMV-encoded proteins. All of these tests were negative for CMV.
The prospective arm consisted of tissue and blood samples from incident cases of HGG. CMV DNA was found in only 3 baseline plasma samples and 1 follow-up sample. 8 out of 15 patients were seropositive, which is similar to the prevalence in the general population. All tissue samples were negative for CMV by a number of methods.
Holdhoff M, Guner G, Rodriguez FJ, et al. "Absence of cytomegalovirus in glioblastoma and other high-grade gliomas by real-time PCR, immunohistochemistry and in situ hybridization." Clinical Cancer Research. 2016. doi: 10.1158/1078-0432.CCR-16-1490