Tuesday, March 7, 2017

Study Finds No Evidence of CMV in High-Grade Gliomas/Glioblastomas

Previous reports on the presence of cytomegalovirus (CMV) in high-grade gliomas (HGG)/glioblastomas (GBM) have been conflicting. A study published in Clinical Cancer Research did not find an association. 

The study consisted of two arms. The retrospective arm consisted of 25 fresh frozen tissues from GBM patients that were tested for CMV by real-time PCR. Tissue microarrays from 70 HGG patients were tested by immunohistochemistry (IHC) for the presence of CMV proteins. 20 formalin-fixed paraffin-embedded GBM tissues were tested by IHC and chromogenic in situ hybridization, targeting specific CMV-encoded proteins. All of these tests were negative for CMV. 

The prospective arm consisted of tissue and blood samples from incident cases of HGG. CMV DNA was found in only 3 baseline plasma samples and 1 follow-up sample. 8 out of 15 patients were seropositive, which is similar to the prevalence in the general population. All tissue samples were negative for CMV by a number of methods.

-Sally Tran

Holdhoff M, Guner G, Rodriguez FJ, et al. "Absence of cytomegalovirus in glioblastoma and other high-grade gliomas by real-time PCR, immunohistochemistry and in situ hybridization." Clinical Cancer Research. 2016. doi: 10.1158/1078-0432.CCR-16-1490

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