Princeton University Researchers have developed a technique to track the replication of Yellow Fever Virus. The method, developed by Alexander Ploss and colleagues, tracks the synthesis of viral RNA in cells as a marker of viral presence.
Using this method, dubbed "RNA Prime Flow", was used to determine that the deletion of the STAT1 gene in mice, involved in interferon signalling, would cause even the vaccine strain of the virus to become virulent. Researchers also used the method to discern that Yellow Fever changes the sorts of cells it infects during the course of infection, moving into B lymphocytes and natural killer cells, where the presence of YF virus had not been observed previously.
https://www.sciencedaily.com/releases/2017/03/170314081601.htm
http://www.nature.com/articles/ncomms14781
-Muzz
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