A team from Harvard created a new technique to actually visualize the process of viral assembly. They decided to study a relatively simple ssRNA virus that infects E. Coli and it has one segment of 3600 nucleotides and a 180-protein capsid. The virus was visualized using interferometric scattering microscopy, where light is scattered off an object that appears as a dark spot. The researchers attached the viral RNA onto a substrate, and as the dark spots grew, they could view how the virus’s size changed over time and even calculate the number of proteins that accumulated. While the viruses assembled over different rates, the one constant was the irreversibility of the process: once the proteins began to join together, there was no turning back or termination of assembly. The data enabled the researchers to propose a model where a critical “nucleus” of proteins forms first before the rest of the capsid can form. The next stage of research is to determine how those proteins actually come together and what potentially regulates the ‘spontaneous’ process of viral assembly.
Watch the virus assemble for yourself here: https://scitechdaily.com/watch-the-very-first-video-of-viruses-assembling/
~Avi Kaye
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